PCR-Free Gene Expression Analysis

PCR-Free Gene Expression Analysis

Sponsor: Affymetrix

The QuantiGene® Singleplex Assay is PCR-free gene expression measurement system that is being extended to include a 384-well format that is easily automatable for high-throughput compound screening. This high-sensitivity assay measures gene expression with minimal sample preparation, using branched DNA for signal amplification. A key feature of the assay is that it does not require RNA or DNA purification, reverse transcription, or PCR amplification, so biases and sample losses resulting from these preparation steps are eliminated. Ideal applications for this assay include: compound screening in drug discovery, biomarker validation, and siRNA knockdown studies. Additionally, the assay accommodates a range of sample types, including heavily degraded and cross-linked RNA in FFPE samples and RNA in blood.