This new range of cancer cell-line models and culture media for the most difficult to culture tumor types where no models may exist, will allow researchers to advance precision medicine initiatives. Directly derived from patient tumors without any genetic manipulation, these cell lines provide the assurance of primary cells with long-term reproducibility and scalability. Unlike traditional protocols for cell-line creation, these cancer models eliminate the possibility of large-scale cellular adaptation through culture and genetic drift. Moreover, these cancer cell models are stable and show a high level of agreement to the original tumor genotype through 150 passages via SNP analysis. The quality control of the cancer cell models are screened for all major categories of cell contaminants, including mycoplasma, microbial contaminants, viruses, cell-line cross-contamination, and STR profiling.