Bio-Rad Laboratories launched the Reliance One-Step Multiplex Supermix, a ready-to-use, single-tube reagent mix optimized for sensitive amplification of up to five targets in a single reaction.
It is formulated with an enzyme blend including a new Reliance reverse transcriptase and Sso7d fusion polymerase, and an advanced buffer to maximize sensitivity and efficiency in multiplex RT-qPCR reactions. Its extended assembled reaction stability (up to 24 hours) is adapted for high-throughput automated workflows. When used as part of a workflow with Bio-Rad’s CFX Automation System II and PrimePCR Probe Assays, the Reliance One-Step Multiplex Supermix enables researchers to maximize throughput and quality of RT-qPCR data, processing 40 plates or more in 24 hours.
One-step RT-qPCR–based applications are increasingly being used in life science research to quickly quantify genes of interest directly from many RNA samples simultaneously. The applications are used in particular and for virus/pathogen detection and for high-throughput screening.