Sponsor: New England Biolabs
The NEBNext Ultra™ II DNA Library Prep Kit for Illumina® next-generation sequencing systems improves upon its predecessor to produce libraries of both higher quality and higher yield from a variety of sample types, including challenging ones, such as formalin-fixed, paraffin-embedded (FFPE) samples and those containing low amounts (500 picograms) of input DNA. The NEBNext Ultra II workflow combines the end repair and dA-tailing steps and minimizes clean up. The protocol for constructing DNA libraries with Ultra II requires just over 10 minutes hands-on time and can be finished in about 2.5 hours. The protocol is compatible with adaptors and primers from NEB or other sources, and can be used in PCR-free and target-enrichment workflows.